phospho smad2 smad3 thr8  (Bioss)

Journal: Cell Biology and Toxicology

Article Title: Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity

doi: 10.1007/s10565-019-09504-5

Figure Lengend Snippet: The detailed information of antibodies

Article Snippet: phospho-Smad2/Smad3 , bs-8853R , Bioss , Polyclonal.

Techniques:

Journal: Cell Biology and Toxicology

Article Title: Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity

doi: 10.1007/s10565-019-09504-5

Figure Lengend Snippet: Effects of 2D-Exos and 3D-Exos on matrix synthesis and the phenotypic stability of chondrocytes. a The protein and mRNA level of genes associated with matrix synthesis and chondrocytic differentiation (Col II, Aggrecan, Sox 9, MMP 13, ADMST5). b Quantitative analysis of s-GAG (left) and s-GAG/DNA (right) when chondrocyte were stimulated by 10 μg/mL exosomes. c Western blot analysis of TGF-β1, P-SMAD2/3, T-SMAD2/3, and Runx 2 of chondrocytes exposed to exosomes. Data represent mean ± SEM. * p < 0.05, ** p < 0.01, n = 3

Article Snippet: phospho-Smad2/Smad3 , bs-8853R , Bioss , Polyclonal.

Techniques: Western Blot

Journal: Journal of Cellular Physiology

Article Title: The long noncoding RNA NORAD enhances the TGF‐β pathway to promote hepatocellular carcinoma progression by targeting miR‐202‐5p

doi: 10.1002/jcp.27869

Figure Lengend Snippet: miR‐202‐5p, targeted by NORAD, inhibit TGFBR1 and TGFBR2 in hepatocellular carcinoma (HCC) cell lines. (a) Targets prediction of NORAD and miR‐202‐5p. (b,c) miR‐202‐5p mimic and its control were added in HCC cells which were transfected by wild‐type or mutated NORAD, TGFBR1 3′‐UTR and TGFBR2 3′‐UTR. The histogram indicates the luciferase values measured 48 hr after transfection. (d) NORAD knockdown, miR‐202‐5p inhibitor and their controls were added in HCC cells which contained TGFBR1 3′‐UTR and TGFBR2 3′‐UTR. The histogram indicates the luciferase values measured 48 hr after transfection. (e) Messenger RNA levels of TGFBR1 and TGFBR2 were measured after NORAD knockdown, miR‐202‐5p inhibitor and their controls were added. (f) Phosphorylation level of Smad2/3 were tested by western blot analysis. *Statistical significance, p < 0.05; **Statistical significance, p < 0.01. TGFBR: transforming growth factor β receptors; UTR: untranslated region [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: Antibody used were listed below: anti‐phospho‐Smad2/Smad3 (p‐smad2/3; bs‐8853R; Bioss, Beijing, China), anti‐Smad2/3 (8685s; Cell Signaling Technology, Danvers, MA), anti‐GAPDH (10494‐1‐AP; Proteintech, Wuhan, China).

Techniques: Transfection, Luciferase, Western Blot